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In vitro wound-healing activity of Ep EVs-LL37 evaluated using a cell migration assay. LL37, Ep EVs, or Ep EVs-LL37 (5 and 10 µg/mL) were treated to <t>HDF</t> <t>cells</t> and observed the cell migration. ( a ) Representative light microscope images showing the cell-free wound area at different time points. ( b ) Quantification of wound area (%) was performed using ImageJ software. The open wound area (%) was plotted against hours post-treatment (hpt). Data were compared with the negative control (NC) group at each time point and reported as means ± SEM. Statistical analysis was performed using two-way ANOVA followed by Dunnett’s multiple comparison test. (Scale bar = 100 μm; * = p < 0.05. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).
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In vitro wound-healing activity of Ep EVs-LL37 evaluated using a cell migration assay. LL37, Ep EVs, or Ep EVs-LL37 (5 and 10 µg/mL) were treated to HDF cells and observed the cell migration. ( a ) Representative light microscope images showing the cell-free wound area at different time points. ( b ) Quantification of wound area (%) was performed using ImageJ software. The open wound area (%) was plotted against hours post-treatment (hpt). Data were compared with the negative control (NC) group at each time point and reported as means ± SEM. Statistical analysis was performed using two-way ANOVA followed by Dunnett’s multiple comparison test. (Scale bar = 100 μm; * = p < 0.05. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).

Journal: Scientific Reports

Article Title: Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activity

doi: 10.1038/s41598-025-28377-9

Figure Lengend Snippet: In vitro wound-healing activity of Ep EVs-LL37 evaluated using a cell migration assay. LL37, Ep EVs, or Ep EVs-LL37 (5 and 10 µg/mL) were treated to HDF cells and observed the cell migration. ( a ) Representative light microscope images showing the cell-free wound area at different time points. ( b ) Quantification of wound area (%) was performed using ImageJ software. The open wound area (%) was plotted against hours post-treatment (hpt). Data were compared with the negative control (NC) group at each time point and reported as means ± SEM. Statistical analysis was performed using two-way ANOVA followed by Dunnett’s multiple comparison test. (Scale bar = 100 μm; * = p < 0.05. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001).

Article Snippet: A cell migration assay was carried out using HDF cells (PCS-201-010TM, ATCC, Manassas, VA, USA) to assess the wound-healing effects of Ep EVs-LL37, based on the method described by Edirisinghe et al. .

Techniques: In Vitro, Activity Assay, Cell Migration Assay, Migration, Light Microscopy, Software, Negative Control, Comparison